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Journal of Clinical Microbiology, April 2000, p. 1679-1680, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pooling of Urine Specimens for Detection of Asymptomatic Chlamydia trachomatis Infections by PCR in a Low-Prevalence Population: Cost-Saving Strategy for Epidemiological Studies and Screening Programs

Servaas A. Morré,1 Chris J. L. M. Meijer,1 Christian Munk,2 Susanne Krüger-Kjaer,2 Jeanette F. Winther,2 Hans O. Jørgensens,3 and Adriaan J. C. van den Brule1,*

Department of Pathology, Section of Molecular Pathology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands,1 and Danish Cancer Society, Institute of Cancer Epidemiology, Copenhagen,2 and Defence Medical Training Center, Gentofte,3 Denmark

Received 18 November 1999/Returned for modification 8 January 2000/Accepted 29 January 2000

Pooling, in groups of five, of urine specimens from asymptomatically infected men in a population with 4% prevalence, as determined by case finding, is 100% sensitive and specific and results in a 60.5% reduction in the number of tests needed. Pooling of urine specimens in groups of 10 for the estimation of population-based prevalence is 96.1% sensitive and 100% specific and saves 90% of the test costs.

* Corresponding author. Mailing address: Department of Pathology, Section of Molecular Pathology, University Hospital Vrije Universiteit, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands. Phone: 31-20-4440503 or 44023. Fax: 31-20-4442964. E-mail: vandenbrule{at}azvu.nl.

Journal of Clinical Microbiology, April 2000, p. 1679-1680, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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