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Journal of Clinical Microbiology, May 2000, p. 1866-1868, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Performance of the ImmunoCard STAT! E. coli O157:H7 Test for Detection of Escherichia coli O157:H7 in Stools

Andrew Mackenzie,1,* Elaine Orrbine,1 Lucie Hyde,1 Michelle Benoit,1 Frank Chan,1 Choong Park,2 Jessica Alverson,2 Amy Lembke,3 Daryl Hoban,4 and William Kennedy5

Child and Youth Clinical Trials Network, Children's Hospital of Eastern Ontario Research Institute, Ottawa,1 Health Sciences Centre, Winnipeg,4 and Isaak Walton Killam Hospital, Halifax,5 Canada; INOVA Fairfax Hospital, Falls Church, Virginia2; and Scottish Rite Children's Hospital, Atlanta, Georgia3

Received 8 November 1999/Returned for modification 4 January 2000/Accepted 28 February 2000

ImmunoCard STAT! E. coli O157:H7 (Meridian Diagnostics, Inc., Cincinnati, Ohio) is a novel rapid (10-min) test for the presence of Escherichia coli O157:H7 in stools. The test may be performed either directly on stool specimens or on an overnight broth culture of stool. In a multicenter prospective study, 14 of 14 specimens positive by culture for E. coli O157:H7 were positive by the ImmunoCard STAT! O157:H7 test, and there were no false positives from 263 culture-negative specimens. In a retrospective study, the test was positive in 339 (81%) of 417 stored culture-positive specimens and the specificity was 95% (98 of 103 specimens). No false positives were associated with alternate stool pathogens. The ImmunoCard STAT! O157:H7 test has high sensitivity and specificity.

* Corresponding author. Mailing address: Child and Youth Clinical Trials Network, Children's Hospital of Eastern Ontario Research Institute, 401 Smyth Rd., Ottawa, ON K1H 8L1, Canada. Phone: (613) 738-3674. Fax: (613) 738-4800. E-mail: amackenzie{at}cyctn.on.ca.

Journal of Clinical Microbiology, May 2000, p. 1866-1868, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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