This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Villiers, E. P.
Right arrow Articles by Allsopp, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Villiers, E. P.
Right arrow Articles by Allsopp, B. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2000, p. 1967-1970, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Macrorestriction Fragment Profiles Reveal Genetic Variation of Cowdria ruminantium Isolates

E. P. de Villiers,* K. A. Brayton,dagger E. Zweygarth, and B. A. Allsopp

Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa

Received 4 October 1999/Returned for modification 22 November 1999/Accepted 31 January 2000

Macrorestriction profile analysis by pulsed-field gel electrophoresis (PFGE) was used to distinguish between seven isolates of Cowdria ruminantium from geographically different areas. Characteristic profiles were generated for each isolate by using the restriction endonucleases KspI, SalI, and SmaI with chromosomal sizes ranging between 1,546 and 1,692 kb. Statistical analysis of the macrorestriction profiles indicated that all the isolates were distinct from each other; these data contribute to a better understanding of the epidemiology of this pathogen and may be exploited for the identification of genotype-specific DNA probes.

* Corresponding author. Mailing address: Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa. Phone: 27 12 5299385. Fax: 27 12 5299431. E-mail: etienne{at}ovisun.ovi.ac.za.

dagger Present address: Washington State University, Pullman, WA 99164-7040.

Journal of Clinical Microbiology, May 2000, p. 1967-1970, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • de Villiers, E. P., Brayton, K. A., Zweygarth, E., Allsopp, B. A. (2000). Genome size and genetic map of Cowdria ruminantium. Microbiology 146: 2627-2634 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»