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Journal of Clinical Microbiology, May 2000, p. 1981-1983, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sensitive Method for Detection of Human Herpesviruses 6 and 7 in Saliva Collected in Field Studies

Danielle M. Zerr,1,* Meei-Li Huang,2 Lawrence Corey,2,3 Matthew Erickson,3 Heather L. Parker,3 and Lisa M. Frenkel1,2

Departments of Pediatrics1 and Laboratory Medicine,2 University of Washington, and Department of Infectious Diseases, Fred Hutchinson Cancer Research Center,3 Seattle, Washington

Received 27 May 1999/Returned for modification 25 August 1999/Accepted 4 November 1999

To facilitate studies of the epidemiology and natural history of human herpesviruses 6 and 7 in infants, a practical method for collecting and quantifying the DNA of these viruses was developed. Saliva was collected using small strips of filter paper, and virus was detected using a real-time quantitative fluorescent-probe PCR assay. The sensitivity and specificity of this method even after prolonged drying of the specimens compared favorably to those of our traditional method of collecting and assaying saliva.

* Corresponding author. Mailing address: Children's Hospital and Regional Medical Center, CH-32, 4800 Sand Point Way, N.E., Seattle, WA 98105. Phone: (206) 528-5086. Fax: (206) 527-3897. E-mail: zerr{at}u.washington.edu.

Journal of Clinical Microbiology, May 2000, p. 1981-1983, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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