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Journal of Clinical Microbiology, May 2000, p. 2001-2004, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Multiplex PCRs for Identification of Escherichia coli Virulence Genes

M. A. Pass,1,* R. Odedra,2,dagger and R. M. Batt2,Dagger

Department of Physiology and Pharmacology, The University of Queensland, St. Lucia, Queensland, 4072, Australia,1 and Department of Small Animal Medicine and Surgery, The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, AL9 7TA, United Kingdom2

Received 3 August 1999/Returned for modification 8 October 1999/Accepted 13 December 1999

PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.

* Corresponding author. Mailing address: Faculty of Science, University of the Sunshine Coast, Locked Bag No. 4, Maroochydore DC, Queensland, 4558, Australia. Phone: 61 7 5430 2840. Fax: 61 7 5430 2887. E-mail: mpass{at}usc.edu.au.

dagger Present address: Amersham Laboratories, Amersham, Buckinghamshire, HP 7 9LL, England.

Dagger Present address: Waltham Centre for Pet Nutrition, Waltham-on-the-Wolds, Leicestershire, LE 14 4RT, England.

Journal of Clinical Microbiology, May 2000, p. 2001-2004, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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