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Journal of Clinical Microbiology, May 2000, p. 2007-2009, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Direct PCR of Cryptococcus neoformans MATalpha and MATa Pheromones To Determine Mating Type, Ploidy, and Variety: a Tool for Epidemiological and Molecular Pathogenesis Studies

Sudha Chaturvedi,1 Birgit Rodeghier,1 Jinjiang Fan,1 Carol M. McClelland,2 Brian L. Wickes,2 and Vishnu Chaturvedi1,3,*

Mycology Laboratory, Wadsworth Center, New York State Department of Health,1 and Department of Biomedical Sciences, School of Public Health, State University of New York at Albany,3 Albany, New York, and Department of Microbiology, University of Texas Health Sciences Center at San Antonio, San Antonio, Texas2

Received 14 December 1999/Returned for modification 29 December 1999/Accepted 16 February 2000

Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.

* Corresponding author. Mailing address: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12208-2002. Phone: (518) 474-4177. Fax: (518) 486-7971. E-mail: vishnu{at}wadsworth.org.

Journal of Clinical Microbiology, May 2000, p. 2007-2009, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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