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Journal of Clinical Microbiology, June 2000, p. 2081-2086, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

False Molecular Clusters due to Nonrandom Association of IS6110 with Mycobacterium tuberculosis

S. H. Gillespie,* A. Dickens, and T. D. McHugh

Department of Medical Microbiology, Royal Free and University College Medical School, University College London, Royal Free Campus, London NW3 2PF, United Kingdom

Received 8 November 1999/Returned for modification 1 February 2000/Accepted 4 March 2000

We sought to determine whether nonrandom association of IS6110 with Mycobacterium tuberculosis could result in false-positive clustering in unselected collections of isolates. We typed 196 strains of M. tuberculosis from an unselected community-based study in northern Tanzania by IS6110 and polymorphic GC-rich repetitive-sequence (PGRS) methodologies. The strains were analyzed by Gelcompar computer software. Analysis of 13 out of 25 groups showed that isolates with identical IS6110 and PGRS patterns were likely to be the same strain. Some IS6110 groups containing strains with identical PGRS patterns had similar IS6110 patterns that differed only by movement of the element. Isolates assigned to a single group (i.e., group 11) on the basis of sharing an identical IS6110 fingerprint pattern did not share identical PGRS fingerprint patterns. Six out of the nine bands in these isolates were in hot-spot locations, as previously defined. This indicates that nonrandom association may result in false-positive clustering in unselected community-based studies. Only strains with identical PGRS and IS6110 patterns are likely to be recently transmitted.

* Corresponding author. Mailing address: Department of Medical Microbiology, Royal Free & University College Medical School, University College London, Royal Free Campus, Rowland Hill St., London NW3 2PF, United Kingdom. Phone: (44)-171-794-0500. Fax: (44)-171-794-0433. E-mail: stepheng{at}rfhsm.ac.uk.

Journal of Clinical Microbiology, June 2000, p. 2081-2086, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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