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Journal of Clinical Microbiology, June 2000, p. 2122-2127, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Quantitative Cytomegalovirus (CMV) PCR in Plasma and CMV Antigenemia Assay: Clinical Utility of the Prototype AMPLICOR CMV MONITOR Test in Transplant Recipients

Angela M. Caliendo,1,2,* Kirsten St. George,3 Shaw-Yi Kao,4 Jessica Allega,1 Ban-Hock Tan,5,dagger Robert LaFontaine,4 Larry Bui,4 and Charles R. Rinaldo3,6

Clinical Microbiology Laboratory1 and Infectious Diseases Unit,5 Massachusetts General Hospital, Boston, Massachusetts; Department of Pathology, Harvard Medical School, Boston, Massachusetts2; Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania3; Roche Molecular Systems, Pleasanton, California4; and Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania6

Received 1 November 1999/Returned for modification 31 January 2000/Accepted 10 March 2000

The correlation between the prototype AMPLICOR CMV MONITOR test (Roche Molecular Systems), a quantitative PCR assay, and the cytomegalovirus (CMV) pp65 antigenemia assay was evaluated in transplant recipients. Sequential blood specimens were collected on 29 patients (491 specimens), the leukocyte fraction was tested by CMV antigenemia, and quantitative PCR was performed on plasma specimens. None of the 15 patients (242 specimens) who were antigenemia negative were positive for CMV DNA by PCR, and none of these patients developed active CMV disease. There were 14 antigenemia-positive patients, 8 of whom developed active CMV disease. In all patients, there was a good association between the antigenemia and PCR assays. Ganciclovir-resistant virus was isolated from three patients with active CMV disease. These three patients had persistently elevated levels of antigenemia and CMV DNA by PCR when resistance to ganciclovir developed. This standardized, quantitative CMV PCR assay on plasma has clinical utility for the diagnosis of active disease and in monitoring the response to antiviral therapy in transplant recipients.

* Corresponding author. Mailing address: Emory University Hospital, Clinical Labs-F147, 1364 Clifton Rd., NE, Atlanta, GA 30322. Phone: (404) 712-5721. Fax: (404) 712-5567. E-mail: acalien{at}emory.edu.

dagger Present address: Department of Internal Medicine, Singapore General Hospital, Singapore.

Journal of Clinical Microbiology, June 2000, p. 2122-2127, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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