This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Khan, N. A.
Right arrow Articles by Paget, T. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khan, N. A.
Right arrow Articles by Paget, T. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2000, p. 2374-2377, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Isolation of Acanthamoeba-Specific Antibodies from a Bacteriophage Display Library

Naveed A. Khan,1 John Greenman,2 Katherine P. Topping,2 Victoria C. Hough,2 Graham S. Temple,3 and Timothy A. Paget1,*

Department of Biological Science,1 Academic Surgical Unit,2 The University of Hull, and Public Health Lab Hull Royal Infirmary,3 Hull, United Kingdom HU6 7RX

Received 21 December 1998/Returned for modification 20 May 1999/Accepted 22 December 1999

Acanthamoeba causes opportunistic eye infections in humans, which can lead to severe keratitis and may ultimately result in blindness. Current methods for identifying this organism rely on culture and microscopy. In this paper, we describe the isolation of antibody fragments that can be used for the unequivocal identification of Acanthamoeba. A bacteriophage antibody display library was used to isolate antibody fragments that bind specifically to Acanthamoeba. Individual clones were studied by enzyme-linked immunosorbent assay, flow cytometry, and immunofluorescence. Four antibody clones that specifically bind to Acanthamoeba spp. were identified.

* Corresponding author. Mailing address: Department of Biological Sciences, Hardy Building, Cottingham Rd., The University of Hull, Hull HU6 7RX, United Kingdom. Phone: 44-1482-465506. Fax: 44-1482-465458. E-mail: T.A.Paget{at}biosci.hull.ac.uk.

Journal of Clinical Microbiology, June 2000, p. 2374-2377, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Smith, K A, Nelson, P N, Warren, P, Astley, S J, Murray, P G, Greenman, J (2004). Demystified...recombinant antibodies.. J. Clin. Pathol. 57: 912-917 [Abstract] [Full Text]  
  • Marciano-Cabral, F., Cabral, G. (2003). Acanthamoeba spp. as Agents of Disease in Humans. Clin. Microbiol. Rev. 16: 273-307 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»