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Journal of Clinical Microbiology, June 2000, p. 2381-2382, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Direct Evidence by DNA Fingerprinting that Endoscopic Cross-Infection of Helicobacter pylori Is a Cause of Postendoscopic Acute Gastritis

Toshiro Sugiyama,1,* Hiroji Naka,1 Akira Yachi,2 and Masahiro Asaka1

Third Department of Internal Medicine, Hokkaido University School of Medicine,1 and Sapporo Medical University,2 Sapporo, Japan

Received 4 October 1999/Returned for modification 29 January 2000/Accepted 4 April 2000

The DNA fingerprinting of Helicobacter pylori strains in two cases of acute gastritis that occurred after endoscopy was examined. H. pylori was isolated from the stomachs of two patients with acute gastritis and from the stomachs of the patients in whom the same gastrofiberscope had previously been used. The genomic DNA digested with HaeIII was subjected to pulsed-field gel electrophoresis. The corresponding paired electrophoretic patterns were completely identical. These findings provide direct evidence that postendoscopic acute gastritis can be caused by cross-infection with H. pylori via endoscopy.

* Corresponding author. Mailing address: Third Department of Internal Medicine, Hokkaido University School of Medicine, N-15, W-7, Kita-ku, Sapporo 060-8638, Japan. Phone: 81-11-716-1161. Fax: 81-11-706-7867. E-mail: tsugi{at}med.hokudai.ac.jp.

Journal of Clinical Microbiology, June 2000, p. 2381-2382, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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