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Journal of Clinical Microbiology, June 2000, p. 2403-2406, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Epidemiologic Typing of Salmonella enterica Serotype Enteritidis in a Canada-Wide Outbreak of Gastroenteritis due to Contaminated Cheese

Rafiq Ahmed,* Geoff Soule, Walter H. Demczuk, Clifford Clark, Rasik Khakhria, Samuel Ratnam,1 Stephen Marshall,dagger Lai-King Ng, David L. Woodward, Wendy M. Johnson,Dagger and Frank G. Rodgers

National Laboratory for Enteric Pathogens, Bureau of Microbiology, Laboratory Centre for Disease Control, Winnipeg, Manitoba R3E 3R2, and Provincial Public Health Laboratory, St. John's, Newfoundland,1 Canada

Received 30 August 1999/Returned for modification 5 November 1999/Accepted 10 March 2000

A major Canada-wide outbreak of gastroenteritis due to Salmonella enterica serotype Enteritidis phage type (PT) 8 occurred in 1998, and this was traced to contaminated cheese in a commercial lunch pack product. Phage typing and pulsed-field gel electrophoresis linked the clinical and cheese isolates of serotype Enteritidis but failed to differentiate outbreak from nonoutbreak PT 8 strains. Further differentiation was made by biotyping based on melibiose fermentation.

* Corresponding author. Mailing address: National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, The Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, Canada R3E 3R2. Phone: (204) 789-6013. Fax: (204) 789-5012. E-mail: Rafiq_Ahmed{at}hc-sc.gc.ca.

dagger Present address: National Research Council, Institute for Biological Sciences, Ottawa, Canada.

Dagger Present address: Cangene Corp., Winnipeg, Canada.

Journal of Clinical Microbiology, June 2000, p. 2403-2406, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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