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Journal of Clinical Microbiology, June 2000, p. 2429-2433, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid Identification of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation Using Real-Time Fluorescence PCR

Udo Reischl,* Hans-Jörg Linde, Michaela Metz, Birgit Leppmeier, and Norbert Lehn

Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany

Received 22 October 1999/Returned for modification 31 January 2000/Accepted 23 March 2000

A duplex LightCycler PCR assay targeting the mecA gene and a Staphylococcus aureus-specific marker was used to test 165 S. aureus strains and 80 strains of other bacterial species. Within an assay time of 60 min plus 10 min for sample preparation, S. aureus as well as the presence or absence of the mecA gene was correctly identified.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Regensburg, Franz-Josef-Strauß-Allee 11, D-93053 Regensburg, Germany. Phone: 49-941-59609-50. Fax: 49-941-944-6402. E-mail: Udo.Reischl{at}klinik.uni-regensburg.de.

Journal of Clinical Microbiology, June 2000, p. 2429-2433, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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