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Journal of Clinical Microbiology, July 2000, p. 2512-2515, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

An Important Proportion of Genital Samples Submitted for Chlamydia trachomatis Detection by PCR Contain Small Amounts of Cellular DNA as Measured by beta -Globin Gene Amplification

François Coutlée,1,2,3,4,* Manon de Ladurantaye,2 Carole Tremblay,2 Jean Vincelette,1,2,4 Louise Labrecque,1,2,4 and Michel Roger1,2,4

Département de Microbiologie et Immunologie, Université de Montréal,1 Département de Microbiologie Médicale et Infectiologie, Centre Hospitalier de l'Université de Montréal,2 Department of Oncology, McGill University,3 and Centre de Recherche du Centre Hospitalier de l'Université de Montréal,4 Montréal, Québec, Canada

Received 29 November 1999/Returned for modification 11 March 2000/Accepted 13 April 2000

We assessed the quality of genital samples submitted for Chlamydia trachomatis detection by PCR by a second PCR assay for the presence of human beta -globin DNA. Endocervical and urethral samples were first tested by the COBAS AMPLICOR C. trachomatis assay (Roche Diagnostic Systems) with an internal control and were then amplified for the presence of beta -globin DNA with primers PC04 and GH20. Samples that contained inhibitors were retested after dilution 1:10. A total of 407 genital samples (311 endocervical swabs from 311 women and 96 urethral swabs from 95 men and 1 woman) collected over a 1-month period were evaluated. The internal control could not be amplified, despite dilution, from 3 of 23 samples that were retested after dilution because of inhibition, leaving 404 samples that could be analyzed by PCR. Eleven samples tested positive for C. trachomatis. Thirty (7.4%) of the 404 samples were negative for beta -globin. Twelve of the 23 undiluted samples that contained inhibitors tested positive for beta -globin DNA. Amplification of beta -globin DNA in samples submitted for C. trachomatis detection by the COBAS AMPLICOR C. trachomatis assay demonstrated that an important proportion of the samples did not contain cellular DNA. Assessment of the quality of the samples for PCR analysis by beta -globin amplification is feasible but cannot replace use of the internal control.

* Corresponding author. Mailing address: Département de Microbiologie Médicale et Infectiologie, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, 1560 Sherbrooke est, Montréal, Québec, Canada H2L 4M1. Phone: 514-281-6000, ext. 5103. Fax: 514-896-4607. E-mail: francois.coutlee{at}ssss.gouv.qc.ca.

Journal of Clinical Microbiology, July 2000, p. 2512-2515, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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