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Journal of Clinical Microbiology, July 2000, p. 2530-2535, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Recombinant Chimeric Borrelia Proteins for Diagnosis of Lyme Disease

Maria J. C. Gomes-Solecki,1 John J. Dunn,2 Benjamin J. Luft,3 Jonathan Castillo,3,dagger Daniel E. Dykhuizen,4 Xiaohua Yang,3 John D. Glass,1 and Raymond J. Dattwyler3,*

Brook Biotechnologies, Inc., Long Island High Technology Incubator, Stony Brook, New York 117901; Biology Department, Brookhaven National Laboratory, Upton, New York 117932; and Department of Medicine3 and Department of Ecology and Evolution,4 State University of New York at Stony Brook, Stony Brook, New York 11794

Received 24 September 1999/Returned for modification 13 December 1999/Accepted 18 February 2000

Current serologic Lyme disease tests use whole borrelia cells as the source of antigen. These assays are difficult to standardize and to optimize for sensitivity and specificity. To help solve these problems, we constructed a library of recombinant chimeric proteins composed of portions of key antigens of Borrelia burgdorferi. These proteins were then used to develop an enzyme-linked immunosorbent assay. We compared our assay with the most sensitive of three whole-cell borrelia assays. We found that the recombinant assay could detect antibodies significantly better from early Lyme disease sera (P < 0.05), and had the same sensitivity for late Lyme disease sera, as the most sensitive whole-cell borrelia assay. On potentially cross-reactive sera, the recombinant assay was more specific, but not significantly so, than the best whole-cell borrelia assay. Optimization of the recombinant assay offers the potential for a significant improvement in both sensitivity and specificity.

* Corresponding author. Mailing address: Department of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-8161. Phone: (631) 444-2351. Fax: (631) 444-3475. E-mail: RAYD{at}epo.som.sunysb.edu.

dagger Present address: University of Massachusetts, Worcester, Mass.

Journal of Clinical Microbiology, July 2000, p. 2530-2535, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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