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Journal of Clinical Microbiology, July 2000, p. 2743-2745, Vol. 38, No. 7
0095-1137/00/$04.00+0

False-Positive Results for Mycobacterium celatum with the AccuProbe Mycobacterium tuberculosis Complex Assay

Ákos Somoskövi,1,2 Jacqueline E. Hotaling,1 Marie Fitzgerald,1 Vivian Jonas,3 Denise Stasik,1 Linda M. Parsons,1 and Max Salfinger1,4,*

Wadsworth Center, New York State Department of Health,1 and Department of Medicine, Albany Medical College,4 Albany, New York; Department of Respiratory Medicine, Semmelweis University, Budapest, Hungary2; and Gen-Probe Incorporated, San Diego, California3

Received 22 December 1999/Returned for modification 13 February 2000/Accepted 29 March 2000

Mycobacterium celatum type 1 was found to cross-react in the AccuProbe Mycobacterium tuberculosis complex assay. Subsequently, we found a statistically significant increase in the relative light units with lower temperatures, suggesting that it is necessary to perform this AccuProbe assay at between 60 and 61°C. We also recommend the inclusion of M. celatum type 1 as a negative control.

* Corresponding author. Mailing address: Wadsworth Center, New York State Department of Health, P.O. Box 509, Albany, NY 12201-0509. Phone: (518) 474-2196. Fax: (518) 474-6964. E-mail: salfinger{at}wadsworth.org.

Journal of Clinical Microbiology, July 2000, p. 2743-2745, Vol. 38, No. 7
0095-1137/00/$04.00+0


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