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Journal of Clinical Microbiology, July 2000, p. 2746-2749, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

PCR Amplification and Phylogenetic Analysis of groESL Operon Sequences from Ehrlichia ewingii and Ehrlichia muris

John W. Sumner,1,* Gregory A. Storch,2 Richard S. Buller,2 Allison M. Liddell,2 Steven L. Stockham,3 Yasuko Rikihisa,4 Sharon Messenger,1 and Christopher D. Paddock1

Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia1; The Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine and St. Louis Children's Hospital, St. Louis, Missouri2; Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, Missouri3; and Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio4

Received 3 January 2000/Returned for modification 21 March 2000/Accepted 19 April 2000

Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.

* Corresponding author. Mailing address: Viral and Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., MS/G13, Atlanta, GA 30033. Phone: (404) 639-1097. Fax: (404) 639-4436. E-mail: jws3{at}cdc.gov.

Journal of Clinical Microbiology, July 2000, p. 2746-2749, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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