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Journal of Clinical Microbiology, July 2000, p. 2778-2780, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of Ehrlichia chaffeensis by Nested PCR in Ticks from Southern China

Wu-Chun Cao,1,* Yu-Min Gao,1 Pan-He Zhang,1 Xi-Tan Zhang,1 Qing-Hua Dai,1 J. Stephen Dumler,2 Li-Qun Fang,1 and Hong Yang1

Institute of Microbiology and Epidemiology, Beijing 100071, China,1 and Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland2

Received 29 November 1999/Returned for modification 21 February 2000/Accepted 1 May 2000

A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55.2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.

* Corresponding author. Mailing address: Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da-Jie St., Fengtai District, Beijing 100071, People's Republic of China. Phone: (086) 10-63862060. Fax: (086) 10-63825138. E-mail: caowc{at}nic.bmi.ac.cn.

Journal of Clinical Microbiology, July 2000, p. 2778-2780, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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