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Journal of Clinical Microbiology, August 2000, p. 2870-2872, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Significant Bacteriuria by Automated Urinalysis Using Flow Cytometry

Hiroshi Okada,1,* Yutaka Sakai,1 Shigenori Miyazaki,1 Soichi Arakawa,1 Yukio Hamaguchi,2 and Sadao Kamidono1

Department of Urology, Kobe University School of Medicine,1 and The Sysmex Corp.,2 Kobe, Japan

Received 3 January 2000/Returned for modification 18 March 2000/Accepted 29 May 2000

A new flow cytometry-based automated urine analyzer, the UF-50, was evaluated for its ability to screen urine samples for significant bacteriuria. One hundred eighty-six urine specimens from patients attending an outpatient clinic of a university-based hospital were examined. The results obtained with the UF-50 were compared with those obtained by conventional quantitative urine culture. The UF-50 detected significant bacteriuria with a sensitivity of 83.1%, a specificity of 76.4%, a positive predictive value of 62.0%, a negative predictive value of 90.7%, and an accuracy of 78.5%. These results are comparable to those obtained by previously reported screening procedures. Besides detecting significant bacteriuria, the UF-50 can also perform routine urinalysis, including measurement of concentrations of red blood cells, white blood cells, epithelial cells, and casts, within 70 s. This capability renders this new flow cytometry-based urine analyzer superior to previously reported rapid screening methods.

* Corresponding author. Mailing address: Department of Urology, Kobe University School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. Phone: 81 78 382 6155. Fax: 81 78 382 6169. E-mail: okada{at}med.kobe-u.ac.jp.

Journal of Clinical Microbiology, August 2000, p. 2870-2872, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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