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Journal of Clinical Microbiology, August 2000, p. 2985-2988, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Outbreak of Methicillin-Resistant Staphylococcus aureus with Reduced Susceptibility to Glycopeptides in a Parisian Hospital

François Guerin,1 Annie Buu-Hoï,1 Jean-Luc Mainardi,1,2 Guillaume Kac,1 Nathalie Colardelle,1 Sabine Vaupré,1 Laurent Gutmann,1,2 and Isabelle Podglajen1,2,*

Laboratoire de Microbiologie Médicale, Hôpital Broussais,1 and Laboratoire de Recherche Moléculaire sur les Antibiotiques, UFR Broussais-Hôtel Dieu et Pitié-Salpétrière,2 Université Paris VI, Paris, France

Received 9 February 2000/Returned for modification 18 March 2000/Accepted 27 May 2000

Epidemiological relationships were investigated between 40 methicillin-resistant Staphylococcus aureus (MRSA) strains with decreased glycopeptide susceptibility isolated from November 1998 to March 1999 from 39 patients (17 infected and 22 colonized patients) in nine wards of the Broussais Hospital, Paris, France. Reduced glycopeptide susceptibility was readily detected on brain heart infusion (BHI) agar containing 6 µg of teicoplanin per ml and on gradient plates, but not by the standard disk diffusion method. The MICs of vancomycin and teicoplanin, determined on BHI agar, were 4 and 8 to 32 µg/ml, respectively (standard antibiotic dilution), and 4 to 8 and 8 to 32 µg/ml, respectively (E-test). All strains were resistant to macrolides, aminoglycosides, tetracycline, rifampin, sulfonamides, and pefloxacin, showed reduced susceptibility to fusidic acid and fosfomycin, and were susceptible to trimethoprim and chloramphenicol. Pulsed-field gel electrophoresis and lysotyping revealed that a multidrug-resistant MRSA clone with decreased susceptibility to glycopeptides has been discretely endemic since at least 1996 in our institution, where it was responsible for an outbreak in November and December 1998.

* Corresponding author. Mailing address: L.R.M.A., Université Paris VI, 15 rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France. Phone: 33-1-01.42.34.68.65. Fax: 33-1-01.43.25.68.12. E-mail: ipodgla{at}ccr.jussieu.fr.

Journal of Clinical Microbiology, August 2000, p. 2985-2988, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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