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Journal of Clinical Microbiology, August 2000, p. 3043-3047, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Identification of Dialister pneumosintes in Subgingival Plaque of Humans

N. Doan,1 A. Contreras,1,2 J. Flynn,1 J. Slots,1 and C. Chen1,*

Department of Periodontology, School of Dentistry, University of Southern California, Los Angeles, California,1 and Department of Periodontology, School of Dentistry, University of Valle, Cali, Colciencias, Colombia2

Received 10 January 2000/Returned for modification 14 March 2000/Accepted 28 April 2000

Dialister pneumosintes is a nonfermentative, anaerobic, gram-negative rod that grows with small, circular, transparent, shiny, smooth colonies on blood agar. Even though D. pneumosintes has been recovered from deep periodontal pockets, little is known about the relationship between the organism and destructive periodontal disease. This study describes a rapid PCR method to identify D. pneumosintes in periodontal samples. The PCR identification method detected as little as 10 pg of D. pneumosintes DNA or about 1 to 10 cells without nonspecific amplification of various periodontopathic bacteria. Twelve of 22 subgingival samples from adult periodontitis lesions yielded D. pneumosintes either by culture or by PCR identification. In culture-positive samples, D. pneumosintes averaged 3.9% (0.001 to 10.8%) of total isolates. Studies are needed to delineate virulence factors of D. pneumosintes pertinent to periodontal disease.

* Corresponding author. Mailing address: University of Southern California, School of Dentistry, Department of Periodontology, Los Angeles, CA 90089-0641. Phone: (213) 740-1075. Fax: (213) 740-2194. E-mail: ccchen{at}hsc.usc.edu.

Journal of Clinical Microbiology, August 2000, p. 3043-3047, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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