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Journal of Clinical Microbiology, August 2000, p. 3076-3079, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid Detection of Campylobacter jejuni in Stool Specimens by an Enzyme Immunoassay and Surveillance for Campylobacter upsaliensis in the Greater Salt Lake City Area

Musa Hindiyeh,1,2 Sandra Jense,1 Sheri Hohmann,1 Hilary Benett,1 Cheryl Edwards,3 William Aldeen,1 Ann Croft,1 Judy Daly,2,4 Susan Mottice,5 and Karen C. Carroll1,2,*

ARUP Laboratories Inc.,1 Department of Pathology, University of Utah Health Sciences Center,2 Primary Children's Medical Center,4 Utah Public Health Laboratory,5 and Cottonwood Hospital Medical Center,3 Salt Lake City, Utah

Received 2 March 2000/Returned for modification 12 April 2000/Accepted 8 May 2000

The Alexon-Trend, Inc. (Ramsey, Minn.), ProSpecT Campylobacter microplate assay was compared with culture on a Campy-CVA plate (Remel, Lenexa, Kans.) and blood-free campylobacter agar with cefoperazone (20 µg/ml), amphotericin B (10 µg/ml), and teicoplanin (4 µg/ml) (CAT medium; Oxoid Limited, Hampshire, England) with 631 patient stool samples. The CAT medium was used to isolate Campylobacter upsaliensis. The enzyme immunoassay (EIA) had a sensitivity and a specificity of 89 and 99%, respectively, and the positive and negative predictive values were 80 and 99%, respectively. Even though we extensively looked for C. upsaliensis in stool samples from patients from the greater Salt Lake City area, we did not isolate this species during the study period. The overall excellent specificity of the EIA allows rapid detection and treatment of positive patients; however, a negative result should be confirmed by culture when clinical suspicion is high.

* Corresponding author. Mailing address: ARUP Laboratories, Medical Director Area, 500 Chipeta Way, SLC, UT 84108, Phone: (801) 583-2787. Fax: (801) 583-2712. E-mail: carrolkc{at}arup-lab.com.

Journal of Clinical Microbiology, August 2000, p. 3076-3079, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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