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Journal of Clinical Microbiology, August 2000, p. 3096-3097, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Use of 5-Bromo-4-Chloro-3-Indolyl-alpha -D-N-Acetylneuraminic Acid in a Novel Spot Test To Identify Sialidase Activity in Vaginal Swabs from Women with Bacterial Vaginosis

R. Wiggins,1 T. Crowley,2 P. J. Horner,2 P. W. Soothill,3 M. R. Millar,4 and A. P. Corfield1,*

Dorothy Crowfoot Hodgkin Laboratories1 and Milne Genito-Urinary Medicine Clinic,2 Division of Medicine, Bristol Royal Infirmary, Bristol BS2 8HW, Fetal Medicine Research Unit, Department of Obstetrics and Gynaecology, St Michael's Hospital, Bristol BS2 8EG,3 and Department of Microbiology, Homerton Hospital, Hackney, London E9 6SR,4 United Kingdom

Received 28 February 2000/Returned for modification 13 April 2000/Accepted 16 May 2000

The validity of measuring vaginal sialidase activity to identify bacterial vaginosis (BV) was determined by using 5-bromo-4-chloro-3-indolyl-alpha -D-N-acetylneuraminic acid in a near-patient test. The sensitivity and specificity of the test for prediction of BV were 95.6 and 96.3%, respectively. Positive and negative predictive values were 95.6 and 96.3%, respectively. This test may be an alternative to Gram staining.

* Corresponding author. Mailing address: Division of Medicine Laboratories, Lower Maudlin St., Bristol Royal Infirmary, Bristol BS2 8HW, United Kingdom. Phone: 0044 (117) 9284898. Fax: 0044 (117) 9283315. E-mail: Tony.Corfield{at}bristol.ac.uk.

Journal of Clinical Microbiology, August 2000, p. 3096-3097, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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