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Journal of Clinical Microbiology, August 2000, p. 3112-3115, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Utility of Commercial Systems for Identification of Burkholderia cepacia Complex from Cystic Fibrosis Sputum Culture

Deborah Blecker Shelly,1 Theodore Spilker,2 Edward J. Gracely,3 Tom Coenye,4 Peter Vandamme,4 and John J. LiPuma2,*

Clinical Microbiology Laboratory, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 191041; Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 481092; Department of Community and Preventive Medicine, MCP Hahnemann University, Philadelphia, Pennsylvania 191293; and Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium4

Received 5 April 2000/Returned for modification 21 May 2000/Accepted 31 May 2000

Performances of several commercial test systems were reviewed to determine their relative levels of accuracy in identifying Burkholderia cepacia complex isolates recovered from cystic fibrosis sputum culture. Positive predictive values ranged from 71 to 98%; negative predictive values ranged from 50 to 82%. All systems misidentified B. cepacia complex. The species most frequently misidentified as B. cepacia was Burkholderia gladioli. These data support the results of previous studies that recommend confirmatory testing, including the use of DNA-based methods, for sputum culture isolates presumptively identified as B. cepacia.

* Corresponding author. Mailing address: Department of Pediatrics and Communicable Diseases, University of Michigan, 1150 W. Medical Center Dr., 8323 MSRB III, Box 0646, Ann Arbor, MI 48109-0646. Phone: (734) 936-9767. Fax: (734) 764-4279. E-mail: jlipuma{at}umich.edu.

Journal of Clinical Microbiology, August 2000, p. 3112-3115, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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