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Journal of Clinical Microbiology, September 2000, p. 3370-3374, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Characterization of Rotavirus in Ireland: Detection of Novel Strains Circulating in the Population

F. O'Halloran,1 M. Lynch,2,3 B. Cryan,2 H. O'Shea,4 and S. Fanning1,*

Molecular Diagnostics Unit1 and Department of Biological Sciences,4 Cork Institute of Technology, Bishopstown, and Department of Medical Microbiology, Cork University Hospital, Wilton,2 Cork, Ireland, and Viral Gastroenteritis Section, MS G-04, Centers for Disease Control and Prevention, Atlanta, Georgia 303333

Received 10 April 2000/Returned for modification 31 May 2000/Accepted 12 July 2000

A collection of three hundred thirty rotavirus-positive stool samples from children with diarrhea in the southern and eastern regions of Ireland between 1997 and 1999 were submitted to the Molecular Diagnostics Unit of the Cork Institute of Technology, Cork, Ireland, for investigation. These strains were characterized by several methods, including polyacrylamide gel electropherotyping and G and P genotyping. A subset of the G types was confirmed by nucleic acid sequencing. The most prevalent types found in this collection included G1P[8] (n = 106; 32.1%), G2P[4] (n = 94; 28.5%), and G4P[8] (n = 37; 11.2%). Novel strains were also detected, including G1P[4] (n = 19; 5.8%), and G4P[4] (n = 2; 0.6%). Interestingly, mixed infections accounted for 18.8% (n = 62) of the total collection, with only 3% (n = 10) which were not G and/or P typeable. Significantly, six G8 and five G9 strains were identified as part of mixed infections. These strains have not previously been identified in Irish children, suggesting a greater diversity in rotavirus strains currently circulating in Ireland.

* Corresponding author. Mailing address: Molecular Diagnostics Unit, Cork Institute of Technology, Bishopstown, Cork, Ireland. Phone: (353-21) 432 6306. Fax: (353-21) 432 6851. E-mail: sfanning{at}cit.ie.

Journal of Clinical Microbiology, September 2000, p. 3370-3374, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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