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Journal of Clinical Microbiology, September 2000, p. 3415-3419, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Distribution of Rotavirus VP7 Serotypes and VP4 Genotypes Circulating in Sousse, Tunisia, from 1995 to 1999: Emergence of Natural Human Reassortants

Abdelhalim Trabelsi,1 Ina Peenze,2 Cara Pager,2 M. Jeddi,1 and Duncan Steele2,*

Laboratoire des Microbiologie-Immunologie, Hopital Universitaire, Farhat Hached, Sousse, Tunisia,1 and MRC/MEDUNSA Diarrhoeal Pathogens Research Unit, Medical University of Southern Africa, Pretoria, South Africa2

Received 22 November 1999/Returned for modification 18 February 2000/Accepted 26 June 2000

Rotavirus strains circulating in Sousse, Tunisia, between 1995 and 1999 were characterized antigenically by monoclonal antibodies to the VP6 subgroup and the VP7 serotype. The VP4 genotype was determined by reverse transcription-PCR, as were the strains with untyped VP7. Only 17% of 375 children were shedding rotavirus as determined by latex agglutination assay. Most rotavirus strains were G1P[8] (50%), followed by G4P[8] and G4P[6]. Reassortant G1P[4] strains emerged in Sousse during the 1998-1999 season.

* Corresponding author. Mailing address: MRC/MEDUNSA Diarrhoeal Pathogens Research Unit, P. O. Box 173, MEDUNSA 0204, Pretoria, South Africa. Phone: (27 12) 521 5938. Fax: (27 12) 521 5794. E-mail: dsteele{at}virol1.medunsa.ac.za.

Journal of Clinical Microbiology, September 2000, p. 3415-3419, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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