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Journal of Clinical Microbiology, September 2000, p. 3498-3501, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genetic Classification of "Rickettsia heilongjiangii" and "Rickettsia hulinii," Two Chinese Spotted Fever Group Rickettsiae

J. Z. Zhang,1,2 M. Y. Fan,2 Y. M. Wu,3 P. E. Fournier,1 V. Roux,1 and D. Raoult1,*

Unité des Rickettsies, Faculté de Médecine, CNRS UPRES-A6020, 13385 Marseille Cedex 05, France,1 and Department of Rickettsiology, Institute of Epidemiology & Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206,2 and Department of Microbiology, Institute of Military Medicine, Shengyang Military Command, Shengyang 110034,3 Peoples Republic of China

Received 24 January 2000/Returned for modification 2 May 2000/Accepted 5 July 2000

To determine the phylogenetic position of two new rickettsial strains isolated from ticks in China, 16S ribosomal DNA, gltA, and ompA (apart from the tandem repeat units) genes were amplified by PCR and sequenced. The phylogenetic relationships between these strains and other rickettsiae were inferred from the comparison of sequences of the three genes by the parsimony, neighbor-joining, and maximum-likelihood methods. The results demonstrated that the 054 strain, a rickettsia pathogenic in humans, and the HL-93 strain were related and clustered together with Rickettsia japonica. Significant statistical bootstrap values (100 and 92%) supported the nodes in this cluster. Based on previous genotypic and antigenic data and the phylogenetic analysis presented here, the 054 and HL-93 strains should be considered as new species, and we formally propose that they be named "Rickettsia heilongjiangii" and "Rickettsia hulinii," respectively.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, CNRS UPRES-A6020, 13385 Marseille Cedex 05, France. Phone: (33) 491 32 43 75. Fax: (33) 491 83 03 90. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, September 2000, p. 3498-3501, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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