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Journal of Clinical Microbiology, September 2000, p. 3515-3517, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of Mycobacterium neoaurum Isolated from a Neutropenic Patient with Catheter-Related Bacteremia by 16S rRNA Sequencing

Patrick C. Y. Woo,1 Hoi-Wah Tsoi,1 Kit-Wah Leung,1 Peggy N. L. Lum,1 Andy S. P. Leung,1 Choi-Ha Ma,2 Kai-Man Kam,2 and Kwok-Yung Yuen1,*

Department of Microbiology, Queen Mary Hospital, The University of Hong Kong,1 and Department of Health,2 Hong Kong, China

Received 28 March 2000/Returned for modification 11 May 2000/Accepted 4 June 2000

A rapidly growing pigmented mycobacterial strain with an ambiguous biochemical profile was isolated from the blood culture taken through the Hickman catheter of a 9-year-old girl with acute lymphoblastic leukemia. Whole-cell fatty acid analysis showed that the best match profile was that of Mycobacterium aurum, but the similarity index was only 0.217, meaning that there were no good matches between the isolate and the organisms in the database of the Microbial Identification System. The 16S rRNA gene of the mycobacterial strain was amplified, agarose gel purified, and sequenced. There were 44 base differences between the gene sequence of the isolate and that of M. aurum but only one base difference between the sequence of the isolate and that of Mycobacterium neoaurum, showing that the isolate was indeed a strain of M. neoaurum by using this "gold standard." This represents the first case of M. neoaurum infection documented by 16S rRNA sequencing.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong, China. Phone: (852) 28553214. Fax: (852) 28551241. E-mail: microgen{at}hkucc.hku.hk.

Journal of Clinical Microbiology, September 2000, p. 3515-3517, Vol. 38, No. 9
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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