This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Desai, M.
Right arrow Articles by Stanley, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Desai, M.
Right arrow Articles by Stanley, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2001, p. 201-206, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.201-206.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Fluorescent Amplified-Fragment Length Polymorphism Subtyping of the Salmonella enterica Serovar Enteritidis Phage Type 4 Clone Complex

Meeta Desai,1 E. John Threlfall,2 and John Stanley1,*

Molecular Biology Unit, Virus Reference Division,1 and Laboratory of Enteric Pathogens,2 Central Public Health Laboratory, London NW9 5HT, United Kingdom

Received 17 July 2000/Returned for modification 1 October 2000/Accepted 28 October 2000

Fluorescent amplified-fragment length polymorphism (FAFLP) analysis, a high-resolution PCR-based genome fingerprinting method, was used to subtype Salmonella enterica serovar Enteritidis phage type 4. This single phage type is responsible for the majority of salmonellosis in Europe. Twenty strains isolated from nine outbreaks, five isolates from sporadic cases of human infection, four strains of poultry origin, and one laboratory-derived strain were comparatively studied by pulsed-field gel electrophoresis (PFGE) and FAFLP analysis. Following macrorestriction with XbaI, PFGE classified 73% of PT4 strains as a single type. FAFLP analysis was carried out with the primer pair EcoRI+0 and MseI+C, by simultaneously sampling 170 to 190 loci throughout the PT4 genome. Twenty-three FAFLP profiles, with 1 to 61 amplified-fragment differences, were found among the 30 strains. The index of discriminatory power of FAFLP analysis was 0.98, compared to 0.47 for PFGE. FAFLP analysis assigned genotypes to each PT4 outbreak, as well as sporadic PT4 infections, a significant development for the epidemiology and control of this zoonotic enteric pathogen.

* Corresponding author. Mailing address: Virus Reference Division, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 0208 200 4400, ext 3090. Fax: 0208 200 1569. E-mail: sevenwoods{at}hotmail.com.

Journal of Clinical Microbiology, January 2001, p. 201-206, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.201-206.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Boxrud, D., Pederson-Gulrud, K., Wotton, J., Medus, C., Lyszkowicz, E., Besser, J., Bartkus, J. M. (2007). Comparison of Multiple-Locus Variable-Number Tandem Repeat Analysis, Pulsed-Field Gel Electrophoresis, and Phage Typing for Subtype Analysis of Salmonella enterica Serotype Enteritidis. J. Clin. Microbiol. 45: 536-543 [Abstract] [Full Text]  
  • Lawson, A. J., Stanley, J., Threlfall, E. J., Desai, M. (2004). Fluorescent Amplified Fragment Length Polymorphism Subtyping of Multiresistant Salmonella enterica Serovar Typhimurium DT104. J. Clin. Microbiol. 42: 4843-4845 [Abstract] [Full Text]  
  • Betancor, L., Schelotto, F., Martinez, A., Pereira, M., Algorta, G., Rodriguez, M. A., Vignoli, R., Chabalgoity, J. A. (2004). Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica Serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002. J. Clin. Microbiol. 42: 1155-1162 [Abstract] [Full Text]  
  • Hu, H., Lan, R., Reeves, P. R. (2002). Fluorescent Amplified Fragment Length Polymorphism Analysis of Salmonella enterica Serovar Typhimurium Reveals Phage-Type- Specific Markers and Potential for Microarray Typing. J. Clin. Microbiol. 40: 3406-3415 [Abstract] [Full Text]  
  • Koort, J. M. K., Lukinmaa, S., Rantala, M., Unkila, E., Siitonen, A. (2002). Technical Improvement To Prevent DNA Degradation of Enteric Pathogens in Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 40: 3497-3498 [Abstract] [Full Text]  
  • Kassama, Y., Rooney, P. J., Goodacre, R. (2002). Fluorescent Amplified Fragment Length Polymorphism Probabilistic Database for Identification of Bacterial Isolates from Urinary Tract Infections. J. Clin. Microbiol. 40: 2795-2800 [Abstract] [Full Text]  
  • Guan, S., Xu, R., Chen, S., Odumeru, J., Gyles, C. (2002). Development of a Procedure for Discriminating among Escherichia coli Isolates from Animal and Human Sources. Appl. Environ. Microbiol. 68: 2690-2698 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»