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Journal of Clinical Microbiology, January 2001, p. 235-240, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.235-240.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Limited Genetic Diversity of Brucella spp.

Benjamín Gándara,1,2 Ahidé López Merino,1 Marco Antonio Rogel,3 and Esperanza Martínez-Romero3,*

Escuela Nacional de Ciencias Biológicas, IPN,1 and Centro de Investigación sobre Fijación de Nitrógeno, UNAM,3 Cuernavaca, and Servicios de Salud de Zacatecas, S.S.A., Zacatecas,2 Mexico

Received 30 June 2000/Returned for modification 29 August 2000/Accepted 16 October 2000

Multilocus enzyme electrophoresis (MLEE) of 99 Brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. In MLEE-derived dendrograms, Brucella abortus and a marine Brucella sp. grouped into a single electrophoretic type related to Brucella neotomae and Brucella ovis. Brucella suis and Brucella canis formed another cluster linked to Brucella melitensis and related to Rhizobium tropici. The Brucella strains tested that were representatives of the six electrophoretic types had the same rRNA gene restriction fragment length polymorphism patterns and identical ribotypes. All 99 isolates had similar chromosome profiles as revealed by the Eckhardt procedure.

* Corresponding author. Mailing address: Centro de Investigación sobre Fijación de Nitrógeno, Ap. Postal 565-A, Av. Universidad S/N, Col. Chamilpa, Cuernavaca, Mexico. Phone: (52-73)-13-16-97. Fax: (52-73)-17-55-81. E-mail: emartine{at}cifn.unam.mx.

Journal of Clinical Microbiology, January 2001, p. 235-240, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.235-240.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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