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Journal of Clinical Microbiology, January 2001, p. 401-403, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.401-403.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Extraction and Genotyping of Cryptosporidium parvum DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination

C. Amar,1,2 S. Pedraza-Díaz,1 and J. McLauchlin1,*

Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT,1 and School of Chemical and Life Sciences, University of Greenwich, Woolwich, London, SE18 8PF,2 United Kingdom

Received 14 August 2000/Returned for modification 11 September 2000/Accepted 23 October 2000

A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.

* Corresponding author. Mailing address: Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, 61 Colindale Ave., London, NW9 5HT, United Kingdom. Phone: 44 20 8200 4400, ext. 3505. Fax: 44 20 8200 8264. E-mail: jmclauchlin{at}phls.nhs.uk.

Journal of Clinical Microbiology, January 2001, p. 401-403, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.401-403.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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  • Amar, C. F.L., Dear, P. H., McLauchlin, J. (2003). Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from human faeces. J Med Microbiol 52: 681-683 [Abstract] [Full Text]  
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