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Journal of Clinical Microbiology, January 2001, p. 86-89, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.86-89.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

International Multicenter Evaluation of Latex Agglutination Tests for Identification of Staphylococcus aureus

Arjanne van Griethuysen,1,* Michèle Bes,2 Jerome Etienne,2 Reinhard Zbinden,3 and Jan Kluytmans4

Department of Clinical Microbiology, St. Elisabeth Hospital, Tilburg,1 and Department of Clinical Microbiology, St. Ignatius Hospital, Breda,4 The Netherlands; Laboratory of Microbiology, Hospital Edouard Herriot, Lyon, France2; and Department of Medical Microbiology, University of Zurich, Zurich, Switzerland3

Received 10 July 2000/Returned for modification 11 August 2000/Accepted 20 October 2000

A newly marketed rapid agglutination kit for the identification of Staphylococcus aureus, Slidex Staph Plus (bioMérieux), was compared to Staphaurex Plus (Murex Diagnostics) and Pastorex Staph-Plus (Sanofi Diagnostics Pasteur). The study took place in three clinical microbiology laboratories in three different European countries. A total of 892 staphylococcal isolates, including 278 methicillin-sensitive S. aureus (MSSA) isolates, 171 methicillin-resistant S. aureus (MRSA) isolates, and 443 coagulase-negative staphylococcal isolates, were analyzed. The sensitivities (MSSA/MRSA) and specificities, respectively, were 98.2% (98.9%/97.1%) and 98.9% for Slidex Staph Plus, 98.2% (98.2%/98.2%) and 96.2% for Staphaurex Plus, and 98.7% (98.6%/98.8%) and 95.7% for Pastorex Staph Plus. The specificity of the Slidex Staph Plus kit was statistically significantly higher than the specificities of Staphaurex Plus and Pastorex Staph-Plus. The Slidex Staph Plus is a very reliable test for the identification of S. aureus.

* Corresponding author. Present address: Department of Medical Microbiology and Medical Immunology, Rijnstate Hospital Arnhem, P.O. Box 9025, 6800 EG Arnhem, The Netherlands. Phone: (31)-26-3788880. Fax: (31)-26-3787279. E-mail: AvanGriethuysen{at}Rijnstate.nl.

Journal of Clinical Microbiology, January 2001, p. 86-89, Vol. 39, No. 1
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.1.86-89.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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