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Journal of Clinical Microbiology, October 2001, p. 3696-3699, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3696-3699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of Efficiency of Screening Extended-Spectrum beta -Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Hospitals Where the Bacteria Are Increasingly Prevalent

K. Lee,1 J. K. Lim,1 D. Yong,1 J. Yum,1 Y. Chong,1,* R. Okamoto,2 and M. Inoue2

Department of Clinical Pathology, Research Institute of Bacterial Resistance, and BK21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea,1 and Department of Microbiology, Kitasato University School of Medicine, Sagamihara City, Japan2

Received 13 February 2001/Returned for modification 10 July 2001/Accepted 29 July 2001

The disk screening methods for extended-spectrum beta -lactamase-producing strains were evaluated. The confirmatory work is reduced significantly in settings such as those in this study, by changing the cefpodoxime breakpoint to <= 20 mm and by not testing cefoxitin-resistant isolates. Cefotaxime and ceftazidime disk screening is reliable, and the laboratory-prepared cefotaxime- and ceftazidime-clavulanic acid disks are stable at -20°C for 12 weeks.

* Corresponding author. Mailing address: Department of Clinical Pathology, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-ku, Seoul, 120-752, Korea. Phone: 82-2-361-5866. Fax: 82-2-313-0908. E-mail: whonetkor{at}yumc.yonsei.ac.kr.

Journal of Clinical Microbiology, October 2001, p. 3696-3699, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3696-3699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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