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Journal of Clinical Microbiology, October 2001, p. 3721-3723, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3721-3723.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Ultrastructural Study of Chlamydia pneumoniae In a Continuous-Infection Model

Andrei Kutlin,1 Cameron Flegg,2 Deb Stenzel,2 Tamara Reznik,1 Patricia M. Roblin,1 Sarah Mathews,2 Peter Timms,2 and Margaret R. Hammerschlag1,*

Department of Pediatrics, State University of New York Downstate Medical Center, Brooklyn, New York,1 and Center for Molecular Biotechnology, Queensland University of Technology, Brisbane, Queensland, Australia2

Received 9 February 2001/Returned for modification 20 June 2001/Accepted 25 July 2001

We have established an in vitro model of long-term continuous Chlamydia pneumoniae infection in HEp-2 cells. Using transmission electron microscopy, we demonstrated the presence of spontaneous abnormal chlamydial inclusions similar in appearance to the persistent chlamydial forms induced in vitro by treatment with cytokines or antibiotics or by nutrient deprivation.

* Corresponding author. Mailing address: Department of Pediatrics, Box 49, SUNY Downstate Medical Center at Brooklyn, 450 Clarkson Ave., Brooklyn, NY 11203-2098. Phone: (718) 245-4075. Fax: (718) 245-2118. E-mail: mhammmerschlag{at}pol.net.

Journal of Clinical Microbiology, October 2001, p. 3721-3723, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3721-3723.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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