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Journal of Clinical Microbiology, October 2001, p. 3764-3767, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3764-3767.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of BACTEC MGIT 960 and BACTEC 460TB Systems for Recovery of Mycobacteria from Clinical Specimens of a University Hospital with Low Incidence of Tuberculosis

Lorenz Leitritz,* Sören Schubert, Bettina Bücherl, Adelheid Masch, Jürgen Heesemann, and Andreas Roggenkamp

Max von Pettenkofer-Institut, 80336 Munich, Germany

Received 31 May 2001/Returned for modification 9 July 2001/Accepted 22 July 2001

Clinical samples obtained over a period of 8 months (n = 2,624) were processed in parallel with the BACTEC 460TB system, with the MGIT 960 system, and in Löwenstein-Jensen (LJ) medium, resulting in the recovery of 127 mycobacteria. Recovery rates in combinations of the BACTEC 460TB or MGIT 960 system with LJ were, respectively, 94.7 and 94.7% for Mycobacterium tuberculosis complex (n = 57) and 91.4 and 70.0% for nontuberculous mycobacteria (n = 70). Contamination rates, elevated in the MGIT 960 system, were associated with patients (cystic fibrosis) and type of material but not with transport time. Detection time was reduced in the MGIT 960 system.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität München, Pettenkoferstr. 9a, 80336 Munich, Germany. Phone: 49-89-5160-5225. Fax: 49-89-5160-4757. E-mail: lorenz.leitritz{at}mvp.med.uni-muenchen.de.

Journal of Clinical Microbiology, October 2001, p. 3764-3767, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3764-3767.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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