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Journal of Clinical Microbiology, October 2001, p. 3768-3771, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3768-3771.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Simple and Rational Approach to the Identification of Mycobacterium tuberculosis, Mycobacterium avium Complex Species, and Other Commonly Isolated Mycobacteria

Derek A. Wong,1,* Peter C. W. Yip,1 Danny T. L. Cheung,2 and Kai Man Kam1

Tuberculosis Reference Laboratory1 and Public Health Laboratory, Kowloon Hospital,2 Pathology Service, Department of Health, HKSAR Government, Hong Kong

Received 6 March 2001/Returned for modification 18 June 2001/Accepted 23 July 2001

A novel PCR-restriction fragment length polymorphism analysis of the hsp65 gene was developed. The restriction patterns for Mycobacterium tuberculosis and Mycobacterium avium complex (MAC) species were designed to be highly distinct, and the overall number of restriction patterns was designed to be limited. Four hundred specimens (17 reference strains and 383 clinical isolates) were tested, of which 98 were M. tuberculosis and 132 were MAC species. The assay was virtually 100% sensitive and specific for M. tuberculosis and MAC species. Moreover, it gave highly concordant results for other mycobacterial species other than M. terrae complex species. This assay can be completed in one day and is user-friendly and robust. Therefore, it is highly suitable for large-scale use in a clinical laboratory.

* Corresponding author. Mailing address: TB Reference Laboratory, 6/F, Yung Fung Shee Memorial Centre, Cha Kwo Ling Rd., Kwun Tong, Hong Kong. Phone: 852 27093742. Fax: 852 29524064. E-mail: derekwong28{at}hotmail.com.

Journal of Clinical Microbiology, October 2001, p. 3768-3771, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3768-3771.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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