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Journal of Clinical Microbiology, October 2001, p. 3778-3780, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3778-3780.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Magnetic Immuno-PCR Assay with Inhibitor Removal for Direct Detection of Helicobacter pylori in Human Feces

Lurdes Monteiro,1,2 Nathalie Gras,1 and Francis Megraud1,*

Laboratoire de Bactériologie, Université Victor Segalen Bordeaux 2, Bordeaux, France,1 and Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisbon, Portugal2

Received 23 February 2001/Returned for modification 30 May 2001/Accepted 23 July 2001

A PCR protocol was developed to detect Helicobacter pylori in human stool specimens. This protocol was based on the association of a magnetic immuno-PCR assay with a technique to remove inhibitors (agarose-embedded DNA preparation). Of the 47 H. pylori-positive and 57 H. pylori-negative patients included in this study, 38 were positive and 66 were negative by this new protocol. The sensitivity, specificity, and predictive values for a positive or a negative result were 80.9% (95% confidence interval [CI], 66.3 to 90.4), 100% (95% CI, 92.1 to 100), 100% (95% CI, 88.6 to 100), and 86.4% (95% CI, 75.2 to 93.2), respectively.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Hôpital Pellegrin, Place Amélie Raba Léon, 33076 Bordeaux Cedex, France. Phone: (33) 5 56 79 59 77. Fax: (33) 5 56 79 60 18. E-mail: francis.megraud{at}chu-bordeaux.fr.

Journal of Clinical Microbiology, October 2001, p. 3778-3780, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3778-3780.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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