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Journal of Clinical Microbiology, October 2001, p. 3796-3798, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3796-3798.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Amino Acid Substitution within the VP7 Protein of G2 Rotavirus Strains Associated with Failure To Serotype

Miren Iturriza Gómara,1,* David Cubitt,2 Ulrich Desselberger,1 and Jim Gray1

Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge CB2 2QW,1 and Camelia Botnar Laboratories, Great Ormond St. Hospital for Children, London WC1N 3JH,2 United Kingdom

Received 15 March 2001/Returned for modification 21 June 2001/Accepted 11 July 2001

Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Aspright-arrowAsn) correlated with the change in ability to serotype these G2 strains.

* Corresponding author. Mailing address: Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Hills Rd., Cambridge CB2 2QW, United Kingdom. Phone: 44-1223-586925. Fax: 44-1223-242775. E-mail: mirren.iturriza-gomara{at}addenbrookes.nhs.uk.

Journal of Clinical Microbiology, October 2001, p. 3796-3798, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3796-3798.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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