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Journal of Clinical Microbiology, October 2001, p. 3799-3800, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3799-3800.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of BacT/Alert 3D Liquid Culture System for Recovery of Mycobacteria from Clinical Specimens Using Sodium Dodecyl (Lauryl) Sulfate-NaOH Decontamination

A. Carricajo,* N. Fonsale, A. C. Vautrin, and G. Aubert

Department of Bacteriology, CHU Bellevue Hospital, Saint-Etienne, France

Received 30 April 2001/Returned for modification 1 July 2001/Accepted 4 August 2001

A total of 52 mycobacterial isolates were recovered from 1,197 clinical specimens decontaminated by a sodium dodecyl (lauryl) sulfate (SDS)-NaOH protocol. Of these, 94% were recovered with the BacT/Alert 3D system (Organon Teknika, Durham, N.C.) and 79% were recovered on Löwenstein-Jensen (LJ) medium. Mean times to detection of organisms of the Mycobacterium tuberculosis complex (n = 47) were 22.8 days with LJ medium and 16.2 days with the system. The BacT/Alert 3D system is a rapid and efficient detection system which can be used with an SDS-NaOH decontamination procedure.

* Corresponding author. Mailing address: Laboratoire de Bactériologie Hôpital Bellevue, CHU Saint Etienne, 42055 Saint Etienne Cedex 02, France. Phone: 4 77 12 77 35. Fax: 4 77 12 05 45. E-mail: anne.carricajo{at}univ-st-etienne.fr.

Journal of Clinical Microbiology, October 2001, p. 3799-3800, Vol. 39, No. 10
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.10.3799-3800.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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