This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pérez-Roth, E.
Right arrow Articles by Méndez-Álvarez, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pérez-Roth, E.
Right arrow Articles by Méndez-Álvarez, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2001, p. 4037-4041, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4037-4041.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Multiplex PCR for Simultaneous Identification of Staphylococcus aureus and Detection of Methicillin and Mupirocin Resistance

E. Pérez-Roth,1 F. Claverie-Martín,1 J. Villar,1 and S. Méndez-Álvarez1,2,*

Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria Hospital,1 and Department of Cellular Biology and Microbiology, University of La Laguna,2 Santa Cruz de Tenerife, Spain

Received 30 July 2001/Returned for modification 20 August 2001/Accepted 4 September 2001

In this work, we describe a multiplex PCR assay for the detection of clinically relevant antibiotic resistance genes harbored by some Staphylococcus aureus isolates and for the simultaneous identification of such isolates at the species level. Conditions were optimized for the simultaneous detection of the 310-, 456-, and 651-bp regions of the mecA (encoding high-level methicillin resistance), ileS-2 (encoding high-level mupirocin resistance), and femB (encoding a factor essential for methicillin resistance) genes, respectively, from a single colony in a single reaction tube. The femB PCR fragment allows the specific identification of S. aureus. Validation of the method was performed using 50 human isolates of methicillin-resistant S. aureus (MRSA) and the appropriate control strains. This assay offers a rapid, simple, feasible, specific, sensitive, and accurate identification of mupirocin-resistant MRSA clinical isolates and could be systematically applied as a diagnostic test in clinical microbiology laboratories, facilitating the design and use of antibiotic therapy.

* Corresponding author. Mailing address: Unidad de Investigación, Hospital Ntra. Sra. de Candelaria, Ctra. del Rosario s/n, 38010 Santa Cruz de Tenerife, Spain. Phone: 34-922-600080 or -600545. Fax: 34-922-600562. E-mail: smendez{at}hcan.rcanaria.es.

Journal of Clinical Microbiology, November 2001, p. 4037-4041, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4037-4041.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hu, D.-L., Omoe, K., Inoue, F., Kasai, T., Yasujima, M., Shinagawa, K., Nakane, A. (2008). Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates. J Med Microbiol 57: 1106-1112 [Abstract] [Full Text]  
  • Ma, S. H., Lee, Y. S., Lee, S. H., Kim, H. K., Jin, J. S., Shin, E. K., Lee, J. C. (2007). Meticillin-resistant Staphylococcus aureus clones with distinct clinical and microbiological features in a Korean community. J Med Microbiol 56: 866-868 [Full Text]  
  • Lemaire, S., Van Bambeke, F., Mingeot-Leclercq, M.-P., Glupczynski, Y., Tulkens, P. M. (2007). Role of Acidic pH in the Susceptibility of Intraphagocytic Methicillin-Resistant Staphylococcus aureus Strains to Meropenem and Cloxacillin. Antimicrob. Agents Chemother. 51: 1627-1632 [Abstract] [Full Text]  
  • Lopez-Aguilar, C., Perez-Roth, E., Mendez-Alvarez, S., Moreno, A., Duran, M. C., Casanova, C., Aguirre-Jaime, A. (2007). Association between the Presence of the Panton-Valentine Leukocidin-Encoding Gene and a Lower Rate of Survival among Hospitalized Pulmonary Patients with Staphylococcal Disease. J. Clin. Microbiol. 45: 274-276 [Full Text]  
  • Perez-Roth, E., Lopez-Aguilar, C., Alcoba-Florez, J., Mendez-Alvarez, S. (2006). High-Level Mupirocin Resistance within Methicillin-Resistant Staphylococcus aureus Pandemic Lineages.. Antimicrob. Agents Chemother. 50: 3207-3211 [Abstract] [Full Text]  
  • Gadepalli, R., Dhawan, B., Sreenivas, V., Kapil, A., Ammini, A.C., Chaudhry, R. (2006). A Clinico-microbiological Study of Diabetic Foot Ulcers in an Indian Tertiary Care Hospital. Diabetes Care 29: 1727-1732 [Abstract] [Full Text]  
  • Cho, D. T., Cha, H. Y., Chang, H.-H., Kim, S.-W., Chung, J. M., Kim, J., Lee, Y. C., Seol, S. Y., Lee, J. C. (2006). Risk factors for specific methicillin-resistant Staphylococcus aureus clones in a Korean hospital. J Antimicrob Chemother 57: 1122-1127 [Abstract] [Full Text]  
  • Zhang, K., Sparling, J., Chow, B. L., Elsayed, S., Hussain, Z., Church, D. L., Gregson, D. B., Louie, T., Conly, J. M. (2004). New Quadriplex PCR Assay for Detection of Methicillin and Mupirocin Resistance and Simultaneous Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci. J. Clin. Microbiol. 42: 4947-4955 [Abstract] [Full Text]  
  • Perez-Roth, E., Lorenzo-Diaz, F., Batista, N., Moreno, A., Mendez-Alvarez, S. (2004). Tracking Methicillin-Resistant Staphylococcus aureus Clones during a 5-Year Period (1998 to 2002) in a Spanish Hospital. J. Clin. Microbiol. 42: 4649-4656 [Abstract] [Full Text]  
  • Paule, S. M., Pasquariello, A. C., Hacek, D. M., Fisher, A. G., Thomson, R. B. Jr, Kaul, K. L., Peterson, L. R. (2004). Direct Detection of Staphylococcus aureus from Adult and Neonate Nasal Swab Specimens Using Real-Time Polymerase Chain Reaction. J. Mol. Diagn. 6: 191-196 [Abstract] [Full Text]  
  • Horii, T., Morita, M., Muramatsu, H., Muranaka, Y., Kanno, T., Maekawa, M. (2003). Effects of mupirocin at subinhibitory concentrations on flagella formation in Pseudomonas aeruginosa and Proteus mirabilis. J Antimicrob Chemother 51: 1175-1179 [Abstract] [Full Text]  
  • Yun, H.-J., Lee, S. W., Yoon, G. M., Kim, S. Y., Choi, S., Lee, Y. S., Choi, E.-C., Kim, S. (2003). Prevalence and mechanisms of low- and high-level mupirocin resistance in staphylococci isolated from a Korean hospital. J Antimicrob Chemother 51: 619-623 [Abstract] [Full Text]  
  • Martin-Lopez, J. V., Perez-Roth, E., Claverie-Martin, F., Diez Gil, O., Batista, N., Morales, M., Mendez-Alvarez, S. (2002). Detection of Staphylococcus aureus Clinical Isolates Harboring the ica Gene Cluster Needed for Biofilm Establishment. J. Clin. Microbiol. 40: 1569-1570 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»