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Journal of Clinical Microbiology, November 2001, p. 4142-4144, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4142-4144.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid and Accurate Determination of Genotypic Clarithromycin Resistance in Cultured Helicobacter pylori by Fluorescent In Situ Hybridization

Holger Rüssmann,1,* Kristin Adler,1 Rainer Haas,1 Bettina Gebert,1 Sibylle Koletzko,2 and Jürgen Heesemann1

Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie1 and Dr. v. Haunersches Kinderspital,2 Ludwig Maximilians-Universität München, 80336 Munich, Germany

Received 23 April 2001/Returned for modification 23 August 2001/Accepted 30 August 2001

Phenotypic susceptibility testing for clarithromycin by E-test and disk diffusion of 109 cultured Helicobacter pylori isolates was compared with the genotypic susceptibility determination by fluorescent in situ hybridization (FISH). No discrepancies were found between these three methods. However, FISH has the advantage of providing results after 3 h.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut, Ludwig Maximilians-Universität, Pettenkoferstr. 9a, 80336 Munich, Germany. Phone: 0049-89-51605261. Fax: 0049-89-51605223. E-mail: ruessmann{at}m3401.mpk.med.uni-muenchen.de.

Journal of Clinical Microbiology, November 2001, p. 4142-4144, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4142-4144.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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