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Journal of Clinical Microbiology, November 2001, p. 4163-4165, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4163-4165.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of a PCR/DNA Probe Colorimetric Membrane Assay for Identification of Campylobacter spp. in Human Stool Specimens

Evelyn Collins,1,* Maura Glennon,1 Shirley Hanley,1 Anne-Marie Murray,2 Martin Cormican,2 Terry Smith,1 and Majella Maher1

The National Diagnostics Centre, BioResearch Ireland, National University of Ireland,1 and Department of Medical Microbiology, University College Hospital,2 Galway, Ireland

Received 15 February 2001/Returned for modification 11 July 2001/Accepted 22 August 2001

DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.

* Corresponding author. Mailing address: DNA Diagnostics Laboratory, The National Diagnostics Centre, National University of Ireland, Galway, Ireland. Phone: 353-91586559. Fax: 353-91586570. E-mail: Evelyn.Collins{at}nuigalway.ie.

Journal of Clinical Microbiology, November 2001, p. 4163-4165, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4163-4165.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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