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Journal of Clinical Microbiology, November 2001, p. 4175-4177, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4175-4177.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Automated Ribotyping to Pulsed-Field Gel Electrophoresis for Genetic Fingerprinting of Streptococcus pneumoniae

John M. Quale, David Landman,* Carlos Flores, and Jayashree Ravishankar

Division of Infectious Diseases, SUNY Downstate Medical Center, Brooklyn, New York 11203

Received 13 June 2001/Returned for modification 25 July 2001/Accepted 16 August 2001

Fifty-two isolates of Streptococcus pneumoniae were characterized by pulsed-field gel electrophoresis (PFGE) and automated ribotyping by using HindIII and PvuII. HindIII ribotypes correlated well with PFGE. PvuII produced fewer bands and was less discriminatory. Automated ribotyping with HindIII is an accurate method for genetic fingerprinting of S. pneumoniae and can complement PFGE.

* Corresponding author. Mailing address: Box 77, SUNY Downstate Medical Center, 450 Clarkson Ave., Brooklyn, NY 11203. Phone: (718) 270-2148. Fax: (718) 270-2465. E-mail: dlandman{at}downstate.edu.

Journal of Clinical Microbiology, November 2001, p. 4175-4177, Vol. 39, No. 11
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.11.4175-4177.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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