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Journal of Clinical Microbiology, November 2001, p. 4181-4183, Vol. 39, No. 11
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.11.4181-4183.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Trends in Antifungal Susceptibility among Swedish
Candida Species Bloodstream Isolates from 1994 to 1998:
Comparison of the E-test and the Sensititre YeastOne Colorimetric
Antifungal Panel with the NCCLS M27-A Reference Method
Erja
Chryssanthou*
Department of Clinical Microbiology,
Karolinska Hospital, Stockholm, Sweden
Received 16 April 2001/Returned for modification 30 July
2001/Accepted 30 August 2001
A comparative evaluation of the NCCLS macrodilution method, the
E-test, and the Sensititre YeastOne Colorimetric Antifungal Panel for
the susceptibility testing of fluconazole, itraconazole, amphotericin
B, and flucytosine was conducted with 233 blood isolates of
Candida species collected between 1994 and 1998 in
Sweden. Antifungal susceptibility profiles of Candida
albicans and non-C. albicans
Candida species remained essentially unchanged within the
5-year study period. The overall agreement rates for the E-test and the
NCCLS MICs and for the YeastOne and the NCCLS MICs were
86 and
87%, respectively, within ±1 dilution for fluconazole, amphotericin
B, and flucytosine, and
66 and
57%, respectively, for
itraconazole. The E-test and the YeastOne panels are equivalent, and
both are convenient methods for routine use.
*
Mailing address: Department of Clinical Microbiology,
L202 Karolinska Hospital, S-171 76 Stockholm, Sweden. Phone: (46)
851773566. Fax: (46) 8308099. E-mail:
Erja.Chryssanthou{at}ks.se.
Journal of Clinical Microbiology, November 2001, p. 4181-4183, Vol. 39, No. 11
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.11.4181-4183.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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