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Journal of Clinical Microbiology, December 2001, p. 4288-4295, Vol. 39, No. 12
Division of Viral and Rickettsial Diseases,
National Center for Infectious Diseases, Centers for Disease Control
and Prevention,1 and Department of
Pediatrics, Emory University,3 Atlanta, Georgia
30333; Atlanta Research and Education Foundation, Decatur,
Georgia 300332; and Osaka City Institute
of Public Health and Environmental Sciences, Osaka, 543, Japan4
Received 5 February 2001/Returned for modification 9 July
2001/Accepted 7 September 2001
"Norwalk-like viruses" (NLVs), members of a newly defined genus
of the family Caliciviridae, are the most common agents
of outbreaks of gastroenteritis in the United States. Two features of
NLVs have hindered the development of simple methods for detection and
determination of serotype: their genetic diversity and their inability
to grow in cell culture. To assess the immune responses of patients
involved in outbreaks of gastroenteritis resulting from infection with
NLVs, we previously used recombinant-expressed capsid antigens
representing four different genetic clusters, but this panel proved
insufficient for detection of an immune response in many patients. To
extend and further refine this panel, we expressed in baculovirus the
capsid genes of three additional genetically distinct viruses, Burwash
Landing virus (BLV), White River virus (WRV), and Florida virus. All
three expressed proteins assembled into virus-like particles (VLPs)
that contained a full-length 64-kDa protein, but both the BLV and WRV
VLPs also contained a 58-kDa protein that resulted from deletion of 39 amino acids at the amino terminus. The purified VLPs were used to
measure the immune responses in 403 patients involved in 37 outbreaks
of acute gastroenteritis. A majority of patients demonstrated a
fourfold rise in the titer of immunoglobulin G to the antigen
homologous to the outbreak strain, but most seroconverted in response
to other genetically distinct antigens as well, suggesting no clear pattern of type-specific immune response. Further study of the antigenicity of the NLVs by use of VLPs should allow us to design new
detection systems with either broader reactivity or better specificity
and to define the optimum panel of antigens required for routine
screening of patient sera.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.12.4288-4295.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Characterization of Capsid Genes, Expressed in the
Baculovirus System, of Three New Genetically Distinct Strains of
"Norwalk-Like Viruses"
*
Corresponding author. Mailing address: Viral
Gastroenteritis Section, Mail Stop G04, Centers for Disease Control and
Prevention, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-2391. Fax: (404) 639-3645. E-mail: smonroe{at}cdc.gov.
Journal of Clinical Microbiology, December 2001, p. 4288-4295, Vol. 39, No. 12
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.12.4288-4295.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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