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Journal of Clinical Microbiology, December 2001, p. 4468-4471, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4468-4471.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Culture with BACTEC Peds Plus/F Bottle Compared with Conventional Methods for Detection of Bacteria in Synovial Fluid

John G. Hughes,1 Emily A. Vetter,1 Robin Patel,1,2 Cathy D. Schleck,3 Scott Harmsen,3 L. Thomas Turgeant,2 and Franklin R. Cockerill III1,2,*

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology,1 Division of Infectious Diseases, Department of Internal Medicine,2 and Department of Health Sciences Research,3 Mayo Clinic, Rochester, Minnesota 55905

Received 11 January 2001/Returned for modification 15 March 2001/Accepted 26 September 2001

An evaluation was undertaken to determine the utility of the BACTEC Peds Plus/F bottle and the BACTEC 9240 instrument (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) for the detection of clinically significant microorganisms in synovial fluid specimens. The Peds Plus/F bottle was used because in our laboratory the quantity of synovial fluid available for culture is frequently in the range of 0.5 to 3.0 ml. The culture results obtained with the Peds Plus/F bottle were compared to those obtained by a conventional agar plate method for a total of 805 synovial fluid specimens. Microbial growth was produced by 74 cultures (9.2%) from 60 patients, yielding a total of 77 microorganisms. Organisms were classified as pathogens (n = 62), contaminants (n = 12), or indeterminate (n = 3) on the basis of a review of the patients' medical histories. Culture using BACTEC Peds Plus/F bottle detected statistically significantly more pathogens overall (62 versus 51 pathogens [P = 0.001]) and statistically fewer contaminants overall (1 versus 11 contaminants [P = 0.006]) than culture by the agar plate method. These results indicate the superior performance of the BACTEC Peds Plus/F bottle over the conventional agar plate method for the detection of clinically significant microorganisms from synovial fluid specimens.

* Corresponding author. Mailing address: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Hilton 470, Mayo Clinic, 200 First St., Rochester, MN 55905. Phone: (507) 284-2901. Fax: (507) 284-4272. E-mail: cockerill.franklin{at}mayo.edu.

Journal of Clinical Microbiology, December 2001, p. 4468-4471, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4468-4471.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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