This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wannet, W. J. B.
Right arrow Articles by Maas, H. M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wannet, W. J. B.
Right arrow Articles by Maas, H. M. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2001, p. 4483-4486, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4483-4486.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Pathogenic Yersinia enterocolitica by a Rapid and Sensitive Duplex PCR Assay

Wim J. B. Wannet,* Michiel Reessink, Henk A. Brunings, and Henny M. E. Maas

National Institute of Public Health and the Environment, Bilthoven, The Netherlands

Received 19 June 2001/Returned for modification 23 July 2001/Accepted 17 September 2001

A duplex PCR assay targeting the ail and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Validation of the assay was performed with 215 clinical Yersinia strains and 40 strains of other bacterial species. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laboratories to identify pathogenic Y. enterocolitica.

* Corresponding author. Mailing address: National Institute of Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA, Bilthoven, The Netherlands. Phone: (31) 30 274 2105. Fax: (31) 30 274 4418. E-mail: wim.wannet{at}rivm.nl.

Journal of Clinical Microbiology, December 2001, p. 4483-4486, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4483-4486.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fredriksson-Ahomaa, M., Korkeala, H. (2003). Low Occurrence of Pathogenic Yersinia enterocolitica in Clinical, Food, and Environmental Samples: a Methodological Problem. Clin. Microbiol. Rev. 16: 220-229 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»