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Journal of Clinical Microbiology, December 2001, p. 4518-4519, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4518-4519.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Automation of Fluorescence-Based PCR for Confirmation of Meningococcal Disease

M. A. Diggle, G. F. S. Edwards, and S. C. Clarke*

Scottish Meningococcus and Pneumococcus Reference Laboratory, Glasgow, United Kingdom

Received 26 June 2001/Accepted 13 September 2001

A fluoresence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningococcus-specific ctrA gene. The method provided a highly sensitive, high-throughput assay that was reproducible and less labor-intensive than manual methods.

* Corresponding author. Mailing address: Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospitals NHS Trust, Department of Microbiology, House on the Hill, Stobhill Hospital, Balornock Rd., Glasgow G21 3UW, United Kingdom. Phone: 44 141 201 3836. Fax: 44 141 201 3836. E-mail: stuart.clarke{at}northglasgow.scot.nhs.uk.

Journal of Clinical Microbiology, December 2001, p. 4518-4519, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4518-4519.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Baethgen, L. F., Moraes, C., Weidlich, L., Rios, S., Kmetzsch, C. I., Silva, M. S.N., Rossetti, M. L.R., Zaha, A. (2003). Direct-test PCR for detection of meningococcal DNA and its serogroup characterization: standardization and adaptation for use in a public health laboratory. J Med Microbiol 52: 793-799 [Abstract] [Full Text]  
  • Smith, K., Diggle, M. A., Clarke, S. C. (2003). Comparison of Commercial DNA Extraction Kits for Extraction of Bacterial Genomic DNA from Whole-Blood Samples. J. Clin. Microbiol. 41: 2440-2443 [Abstract] [Full Text]  
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