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Journal of Clinical Microbiology, December 2001, p. 4529-4531, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4529-4531.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Identification of Methicillin-Resistant Staphylococcus aureus from Positive Blood Cultures by Real-Time Fluorescence PCR

Thean Yen Tan,1,* Sally Corden,2 Rosemary Barnes,1 and Barry Cookson3

Department of Microbiology, Public Health Laboratory Service,1 and Department of Virology, Public Health Laboratory Service,2 Cardiff, and Laboratory of Hospital Infections, Central Public Health Laboratory, London,3 United Kingdom

Received 7 June 2001/Returned for modification 23 July 2001/Accepted 13 September 2001

Methicillin-resistant Staphylococcus aureus septicemia is associated with significant morbidity and mortality and requires treatment with intravenous glycopeptides. For blood cultures positive for gram-positive cocci, 24 to 48 h is required for the detection of S. aureus bacteremia and the provision of antibiotic susceptibility testing results. We describe a molecular biology-based assay that requires 2 h from the time of initial positivity of blood cultures. The assay correctly detected 96% of the S. aureus isolates including all methicillin-resistant S. aureus isolates. Clinical data collected during the study suggest that 28% of patients with S. aureus bacteremia do not receive early and appropriate treatment and that 10% of patients may initially be receiving inappropriate glycopeptide treatment.

* Corresponding author. Mailing address: Department of Microbiology, Cardiff Public Health Laboratory, University Hospital Wales, Cardiff CF14 4XW, United Kingdom. Phone: 44-029-20742718. Fax: 44-029-20742161. E-mail: theanyen.tan{at}phls.wales.nhs.uk.

Journal of Clinical Microbiology, December 2001, p. 4529-4531, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4529-4531.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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