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Journal of Clinical Microbiology, December 2001, p. 4541-4543, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4541-4543.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Association of the Urease Gene with Enterohemorrhagic Escherichia coli Strains Irrespective of Their Serogroups

Masayuki Nakano,1 Tetsuya Iida,1,* Makoto Ohnishi,2 Ken Kurokawa,3 Akira Takahashi,4 Teizo Tsukamoto,5 Teruo Yasunaga,3 Tetsuya Hayashi,2 and Takeshi Honda1

Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University,1 and Genome Information Research Center, Osaka University, Suita, Osaka,3 Department of Microbiology, Miyazaki Medical College, Kiyotake, Miyazaki,2 Department of Nutrition, School of Medicine, Tokushima University, Tokushima, Tokushima,4 and Osaka Prefectural Institute of Public Health, Osaka,5 Japan

Received 24 May 2001/Returned for modification 3 September 2001/Accepted 19 September 2001

Among various diarrheagenic Escherichia coli strains from clinical sources, we found that the urease gene was specifically associated with enterohemorrhagic E. coli (EHEC) strains irrespective of their serogroups. The results suggest that the urease gene can be a useful genetic marker for the detection of EHEC strains and for the diagnosis of infections caused by EHEC strains in the clinical situation.


* Corresponding author. Mailing address: Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-8278. Fax: 81-6-6879-8277. E-mail: iida{at}biken.osaka-u.ac.jp.


Journal of Clinical Microbiology, December 2001, p. 4541-4543, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4541-4543.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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