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Journal of Clinical Microbiology, December 2001, p. 4549-4553, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4549-4553.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Automated Ribotyping Provides Rapid Phylogenetic Subgroup Affiliation of Clinical Extraintestinal Pathogenic Escherichia coli Strains

Olivier Clermont,1 Christophe Cordevant,2 Stephane Bonacorsi,1 Armelle Marecat,2 Marc Lange,2 and Edouard Bingen1,*

Laboratoire d'Études de Génétique Bactérienne dans les Infections de l'Enfant (EA3105), Université Denis Diderot-Paris 7, Hôpital Robert Debré, Paris,1 and Institut Pasteur de Lille, Lille,2 France

Received 5 April 2001/Returned for modification 23 July 2001/Accepted 27 September 2001

Using the automated Riboprinter system, we have initiated the construction of an electronic Riboprint database composed of 72 ECOR reference strains and 15 archetypal virulent strains in order to provide a new simple molecular characterization method. More than 90% of the ECOR strains clustered in their original phylogenetic group. All but one of the archetypal virulent strains had a profile identical to that of one of the ECOR strains and could be easily affiliated with a phylogenetic group. This method appears to be an accurate and practical tool especially for investigating the genetic relationship between clinical extraintestinal pathogenic strains and B2 subgroup ECOR strains or archetypal pathotype strains.

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Robert Debré, 48 Blvd. Sérurier, 75395 Paris cedex 19, France. Phone: 33 1 40 03 23 40. Fax: 33 1 40 03 24 50. E-mail: edouard.bingen{at}rdb.ap-hop-paris.fr.

Journal of Clinical Microbiology, December 2001, p. 4549-4553, Vol. 39, No. 12
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.12.4549-4553.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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